fluorogenic acetylated hdac substrate 3 Search Results


3,30-diaminobenzidine substrate  (Nichirei Biosciences)
90
Nichirei Biosciences 3,30-diaminobenzidine substrate
3,30 Diaminobenzidine Substrate, supplied by Nichirei Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3,3′-diaminodbenzidine (dab) substrate  (Fisher Scientific)
90
Fisher Scientific 3,3′-diaminodbenzidine (dab) substrate
3,3′ Diaminodbenzidine (Dab) Substrate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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supersensitive tmb substrate (3,3’,5,5’-tetramethylbenzidine  (Millipore)
90
Millipore supersensitive tmb substrate (3,3’,5,5’-tetramethylbenzidine
Supersensitive Tmb Substrate (3,3’,5,5’ Tetramethylbenzidine, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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color substrate 3 3 5 5 tetramethylbenzidine  (Millipore)
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Millipore color substrate 3 3 5 5 tetramethylbenzidine
Color Substrate 3 3 5 5 Tetramethylbenzidine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3, 3’-diaminobenzidine liquid substrate  (Agilent technologies)
90
Agilent technologies 3, 3’-diaminobenzidine liquid substrate
3, 3’ Diaminobenzidine Liquid Substrate, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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insulin receptor substrate 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology insulin receptor substrate 1
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
Insulin Receptor Substrate 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3,3′,5,5′-221 tetramethylbenzidine liquid substrate (tmb  (Millipore)
90
Millipore 3,3′,5,5′-221 tetramethylbenzidine liquid substrate (tmb
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
3,3′,5,5′ 221 Tetramethylbenzidine Liquid Substrate (Tmb, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substrate 3 amino 9 ethyl carbazole  (Vector Laboratories)
86
Vector Laboratories substrate 3 amino 9 ethyl carbazole
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
Substrate 3 Amino 9 Ethyl Carbazole, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3 0,3 0-diaminobenzidine  (Agilent technologies)
90
Agilent technologies 3 0,3 0-diaminobenzidine
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
3 0,3 0 Diaminobenzidine, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aec substrate (3-amino-9-ethyl-carbazole  (Millipore)
90
Millipore aec substrate (3-amino-9-ethyl-carbazole
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
Aec Substrate (3 Amino 9 Ethyl Carbazole, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aec substrate (3-amino-9ethyl-carbazole  (Millipore)
90
Millipore aec substrate (3-amino-9ethyl-carbazole
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
Aec Substrate (3 Amino 9ethyl Carbazole, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3,3∞-diaminobenzidine tetrahydrochloride  (Dojindo Labs)
90
Dojindo Labs 3,3∞-diaminobenzidine tetrahydrochloride
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
3,3∞ Diaminobenzidine Tetrahydrochloride, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) Insulin receptor β (IRβ), insulin receptor substrate 1 (IRS1), phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.

Journal: The journals of gerontology. Series A, Biological sciences and medical sciences

Article Title: Differential Development of Inflammation and Insulin Resistance in Different Adipose Tissue Depots Along Aging in Wistar Rats: Effects of Caloric Restriction.

doi: 10.1093/gerona/glv117

Figure Lengend Snippet: Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) Insulin receptor β (IRβ), insulin receptor substrate 1 (IRS1), phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.

Article Snippet: Antibodies directed toward phospho IRβ (Tyr 1162/1163), insulin-Rβ (C-19), insulin receptor substrate 1 (IRS1; C-20), and PTP1B (H135) were from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Phospho-proteomics, Ex Vivo, Saline, Western Blot, Control